How You Will Achieve It  

• Apply technical skills to complete assigned work within own work group/project team. 

• Optimize suspension mammalian cells for transient transfection using DOE principle.

• Monitor and quantify protein expression level in cells by different assays such as Octet, FACS, Fluorescence microscopy, SDS-Page, Western, etc. after transient transfection.

• Generate stable cell lines, primarily in CHO cells, by both targeted and random integration to produce vaccine candidates or antibodies.

• Characterize cell lines with different assays such as ddPCR, NOVA, FACS, Western etc. to measure genetic and epigenetic stability.

• Perform aseptic handling of mammalian cells and prepare cell banks.

• Support laboratory activities by monitoring lab equipment and maintaining a clean and safe working environment. 

• Analyze, interpret, and provide conclusions and present experimental data. Prepare technical reports, as needed.

• Collaborate with colleagues towards introduction of new technologies to improve and accelerate upstream process development and improve process efficiency.

• Present problems and propose solutions in discussion with group members.  Offers insights based on experience to group members to help solve problems. 

• Effectively document all lab activities in notebooks/elsewhere as required and appropriate, in a timely fashion. 

Qualifications  

Must-Have  

• Bachelor’s degree in biology, Molecular Biology, Cell Biology, Microbiology, Biochemistry or related discipline with some related experience.

• A strong background in genetics, molecular biology, cell biology and aseptic cell culture techniques is essential.

• Practical knowledge of mammalian cell culture and metabolism. 

• Team oriented, with excellent oral and written communication skills 

• Well organized and great attention to detail. 

• Proficiency in Microsoft systems such as Office suite, Outlook, Etc.

• Experience in a biochemical laboratory environment.

Nice-to-Have  

• Experience in protein expression by transient transfection in mammalian cells.

• Hands-on experience with mammalian cell culture, cell line development activities that involve introduction of recombinant genes into mammalian genomes is expected. Bioreactor/Wave system operation are a plus.

• The ability to design complex experiments and interpret data. 

• Practical knowledge of bioreactor operations. 

PHYSICAL/MENTAL REQUIREMENTS

• Ability to make aseptic transfers.

• Ability to lift 25lbs.

• Mental requirements are a detailed understanding of the scientific subject matter.

• Ability to make scientific judgments and an ability to perform complex data analysis.

NON-STANDARD WORK SCHEDULE, TRAVEL OR ENVIRONMENT REQUIREMENTS

• The work requires a flexible work schedule to properly perform cell line development manipulations.

• This may involve the necessity of working outside of core hours and on weekends.

Other Job Details:

• Last Date to Apply for Job: August 31st, 2024

• Work Location Assignment: On Premise

  On Premise colleagues work in a Pfizer site because it’s needed to get their job done. They may have flexibility to work remotely from time to time, but they are primarily on-site.

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